Wednesday, August 1, 2018

Amplicon Inactivation

The rapid growth of DNA sequencing in laboratories is resulting in the increased use of PCR readers.  As a result,  researchers are making advances in science and medicine, but not without new challenges. One challenge is that the integrity of the results can be affected if amplicon residues contaminate part of the PCR reader. This contamination could cause improper analysis of subsequent samples. Mitigation of this problem requires amplicons to be inactivated from all surfaces of the PCR reader, including all cracks and crevices of the equipment.

Chlorine dioxide gas cycles have been validated with multiple PCR reader manufacturers in order to clear the reader from residual amplicons which can cause inaccurate results and readings.  The validation consisted of a series of cycles with varying chlorine dioxide gas dosages that were tested to achieve an inactivation of the amplicons. The verification process also confirmed that the equipment was not corroded as a result of the treatment cycles.

ClorDiSys Solutions, Inc performs amplicon inactivation work as part of its contract sterilization and decontamination services.  Two manufacturers of PCR readers have their customers send equipment to our facility before having it returned to the manufacturer for maintenance and repair.  At our facility, our contract sterilization team treats the units to eliminate any residual amplicons and blood-borne pathogens. This treatment allows the equipment to be safely handled by the manufacturers' maintenance department.

For more information, please read our Application Note on Amplicon Inactivation here.

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