The Class III BSC can be decontaminated as part of the room (by opening the gull wing door), or it can be decontaminated on its own through use of the built-in connectors. The components needed are RH probe, mix box (which contains a humidity generator), blower motor, DC/AC controller, pressure relief scrubber, and the Minidox generator. The CD gas concentration is monitored via a gas sample port. This hose is connected to the Minidox, which then, on the basis of real-time readings, activated the gas injection system as needed. The scrubber removes any CD gas during this process. A standard cycle of 5 mg/L for 30 minutes of exposure is often used for Class III BSCs. However, due to the nature of this particular facility’s work, the cycle time was extended to 45 to 60 minutes. All biological indicators (BIs) were repeatedly killed, and no issues of corrosion were evident. All components continue to remain free of any imperfections. Due to that, chlorine dioxide gas is now the method of choice for the decontamination of Class III BSCs.
To read more about Tufts New England Regional Biosafety Laboratory’s utilization of chlorine dioxide gas for both BSC and room decontamination, click here.