The almost 500,000 ft3 Blend Room was turned over to our team at 4pm. At this time, gas injection tubing was run to 10 different locations in order to speed up the natural distribution of the gas. Gas sample tubing was run to 6 different locations within the Blend Room in order to measure gas concentrations throughout the decontamination process. This enables our team to confidently control the process, making sure that the entire room has been subjected to the proper dosage of chlorine dioxide gas necessary to achieve a 6-log sporicidal reduction. Biological Indicators were placed in 20 different locations throughout the space, including placement under trash cans, as well as beneath and behind equipment. Conveyors, doors, and the HVAC system handling the room were then sealed. At approximately 11:15pm, gassing started. At 3:15 am, all areas within the room reached the appropriate dosage, and aeration began. Upon completion, equipment was removed from the space along with biological indicators. The next day, Biological Indicators were dropped in growth media and incubated for 36 hours to check if any of the million bacterial spores contained on the indicator were left viable to grow and multiply. After 36 hours, none of the 20 biological indicators showed any growth, proving that a 6-log sporicidal reduction took place within the Blend Room. The project was successful and the company was able to start production shortly after.
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